point-of-care elisa test snap ® felv antigen test  (IDEXX)

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: Prevalence (in percentage of positive individuals), 95% confidence intervals, and sample size (between brackets) of FeLV p27 ELISA in blood, FeLV PCR proviral DNA in blood and tissues recorded in the free-ranging Iberian lynx population from Andalusia in 2008–2021.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: FeLV prevalence results by age ( a ) and sex ( b ). * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars denote ± SE.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques:

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: Map of Andalusia (southern Spain) displaying a FeLV provirus prevalence gradient reflecting 2008–2021 qPCR results at the municipal level in blue-green as well as nationally protected natural areas in brown. The Sierra Morena (northern range) population encompasses a significant spatial cluster for FeLV provirus-positive Iberian lynxes and domestic cats while the Doñana-Aljarafe population (western range) does not. Red triangles and blue circles indicate FeLV provirus positive and negative lynxes, respectively. Yellow stars and orange diamonds indicate FeLV provirus-positive and negative cats, respectively.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques: Northern Blot, Western Blot

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: FeLV prevalence by year taking into account each of the screening methods used in this study. Letters indicated significant pairwise differences between years ( p < 0.05). Shaded error bar denotes ± SE.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques:

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: ( a ) FeLV prevalence results by population using FeLV proviral DNA in blood and tissue as screening method. Letters indicated significant pairwise differences between years ( p < 0.05). ( b ) FeLV antigenemia prevalence results by population using ELISA p27 as screening method. Shaded error bars denote ± SE.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: FeLV diagnosis by provirus qPCR in longitudinally sampled lynxes.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques: Biomarker Discovery, Sampling

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: FeLV p27 ELISA results obtained from lynxes involved in the 2007 FeLV outbreak.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: FeLV diagnosis results from a subadult male (“Coca”) extracted from the field during the 2007 FeLV outbreak.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx ( Lynx pardinus ) in Andalusia, Spain (2008–2021)

doi: 10.1038/s41598-024-55847-3

Figure Lengend Snippet: Possible outcomes of feline leukemia virus infection detected in this study and associated test results.

Article Snippet: Three drops of whole blood were used in situ to run a fast (10 min) point-of-care (POC) antigen FeLV ELISA test (IDEXX ® Snap test, IDEXX Laboratories, Inc., Maine, USA), which detects the p27 protein of the FeLV capsid in blood.

Techniques: Virus, Infection, Enzyme-linked Immunosorbent Assay